Chromatography and distillation

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Lesson notes

Chromatography

  • Chromatography separates different substances in a mixture.
  • A spot of ink is placed on chromatography paper and the paper is placed in water.
  • Water rises up the paper and acts as a solvent.
  • Ink is made of different dyes that are soluble in water.
  • As water rises, it brings dyes up the paper; different dyes move at different rates.
  • Dyes that travel the same distance up the paper are the same dye.

The three steps of a paper chromatography experiment: spotting the sample on a pencil baseline, lowering the paper into solvent below the baseline, and analysing the separated spots on the chromatogram.

The three steps of a paper chromatography experiment: spotting the sample on a pencil baseline, lowering the paper into solvent below the baseline, and analysing the separated spots on the chromatogram.

Simple Distillation

  • Simple distillation separates a liquid from a dissolved solid (e.g., salt from seawater).
  • A Bunsen burner heats the mixture; the liquid evaporates, leaving the solid behind.
  • Vapour travels through a condenser where cooling water passes.
  • The cool condenser condenses the vapour back to liquid, collected as distilled water.
  • This method can also separate liquids with different boiling points.

Simple distillation of salty water: water vapour rises from the flask, passes through the condenser (cooling water in at the beaker end, out at the flask end), and is collected as pure water.

Simple distillation of salty water: water vapour rises from the flask, passes through the condenser (cooling water in at the beaker end, out at the flask end), and is collected as pure water.

Key Terms

  • Soluble: able to dissolve in a solvent.
  • Solvent: a liquid that dissolves a substance (e.g., water in chromatography).
  • Evaporation: change from liquid to gas.
  • Condensation: change from gas to liquid.

Common Misconceptions

  • In chromatography, ink is not the solvent; water is the solvent.
  • Chromatography separates mixtures, not elements in a compound.
  • Salt cannot be filtered from seawater because salt dissolves; distillation is needed.

Application: Separating Liquids

  • If two liquids have different boiling points, the one with the lower boiling point evaporates first.
  • Example: Liquid A (boiling point 100°C) will evaporate before Liquid B (300°C).

Fractional distillation of a water/ethanol mixture: the fractionating column allows repeated condensation and evaporation so the lower-boiling ethanol distils off first.

Fractional distillation of a water/ethanol mixture: the fractionating column allows repeated condensation and evaporation so the lower-boiling ethanol distils off first.

States of matter: solid, liquid, gas. Evaporation (liquid to gas) and condensation (gas to liquid) are key in distillation.

Particle arrangementSolidLiquidGas

Slides

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Practice questions

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  1. 1.In chromatography, what is the solvent?

    Easy
    • AWater
    • BInk
    • CDye
    • DPaper
  2. 2.Which statement about chromatography is correct?

    Easy
    • ADyes are soluble in the solvent.
    • BThe solvent is the ink.
    • CChromatography separates compounds into elements.
    • DAll dyes move at the same rate.
  3. 3.What is the name of the state change from gas to liquid?

    Easy
    • ACondensation
    • BEvaporation
    • CBoiling
    • DMelting
  4. 4.What is the name of the state change from liquid to gas?

    Easy
    • AEvaporation
    • BCondensation
    • CFreezing
    • DSublimation
  5. 5.In simple distillation of seawater, what is left behind in the flask?

    Easy
    • ASalt
    • BPure water
    • CWater vapour
    • DCondensed water
  6. 6.During simple distillation, what is the purpose of the cooling water in the condenser?

    Easy
    • ATo condense the vapour into liquid
    • BTo evaporate the liquid
    • CTo heat the vapour
    • DTo dissolve the salt
  7. 7.A mixture of two liquids is distilled. Liquid A boils at 100°C, liquid B at 300°C. Which liquid is collected first?

    Easy
    • ALiquid A
    • BLiquid B
    • CBoth together
    • DNeither
  8. 8.Why can't salt be removed from seawater by filtration?

    Easy
    • ASalt particles are too small to be trapped by filter paper
    • BSalt is insoluble in water
    • CFilter paper dissolves in seawater
    • DFiltration only works for gases

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