Mass Transport In Plants
விளையாடிக் கற்றுக்கொள்ளுங்கள்
ஆற்றல் சம்பாதிக்க இந்த கேள்விகளுக்குப் பதிலளியுங்கள், பின்னர் மீன் பிடித்து ஆராயுங்கள். கணக்கு தேவையில்லை.
பாட குறிப்புகள்
Transpiration and the Xylem
- Transpiration is the loss of water from the leaves of plants by evaporation.
- Water and dissolved minerals are transported from the soil to the rest of the plant in the xylem.
- Xylem cells are specialised for water transport: they are hollow tubes with no end walls, allowing continuous flow of water.
- Lignin provides waterproofing to prevent loss of water by evaporation and strengthens the xylem to reduce breakages.
- Xylem tissue, together with phloem tissue, makes up the plant's vascular tissue.
- Water is required for photosynthesis and to maintain cell structure; minerals are needed for production of important biological molecules, e.g. proteins and chlorophyll.
The Cohesion-Tension Theory
- The upward movement of water in the xylem is driven by transpiration.
- Water diffuses out of leaves into the surrounding air via the stomata.
- Loss of water vapour lowers the water potential in the air spaces surrounding the mesophyll cells.
- Water within the mesophyll cell walls evaporates into the leaf air spaces, lowering the water potential of the mesophyll cells.
- Water is drawn from the xylem into the mesophyll cells by osmosis.
- Water moves up the xylem vessels in a continuous column to replace lost water; this upward movement is the transpiration stream.
- Water molecules are pulled upwards due to forces of cohesion (between water molecules) and adhesion (between water and the sides of the xylem).
- The upward pulling force can put water under tension, exerting an inward pull on the walls of the xylem vessels; this is known as cohesion-tension.
Factors Affecting Transpiration Rate
- High wind speed increases transpiration: wind moves water vapour away from the leaf surface, increasing the water potential gradient between the leaf and the air, increasing diffusion of water vapour.
- High humidity decreases transpiration: the water potential gradient between the leaf and the air is reduced, slowing diffusion of water vapour.
- High light intensity increases transpiration: the rate of photosynthesis increases, so stomata open to allow gas exchange, allowing more water to diffuse out.
- High temperature increases transpiration: particles have more kinetic energy so water molecules evaporate from the mesophyll and diffuse out of the leaf more quickly.
Using a Potometer
- A potometer can be used to investigate the effect of environmental factors on the rate of transpiration.
- Cut a plant shoot underwater using a diagonal cut: a diagonal cut creates a larger surface area for the uptake of water.
- Assemble the potometer underwater to prevent air bubbles from entering the xylem where they could block the movement of water.
- Make sure the apparatus is airtight using petroleum jelly to seal any gaps, preventing entry of air into the system.
- Dry the leaves using a paper towel: water on the leaves will block the stomata and affect transpiration.
- Allow a single air bubble to form inside the potometer and place the end of the tube into a beaker of water; the air bubble allows water movement in the tube to be observed, and the beaker provides a supply of water to replace water lost during transpiration.
- Set up the environmental factor to be assessed and allow the plant shoot to adjust to the new environment for 5 minutes.
- Record the starting location of the air bubble, leave for a set period (e.g. 15 minutes), record the end location, then reset the bubble by opening the tap below the reservoir.
- Repeat steps twice more under the same environmental conditions before repeating under changed environmental conditions.
- Note that a potometer measures the rate at which a plant shoot takes up water, not the true rate of transpiration; most of this water is lost in transpiration, but some may enter cells and be used in photosynthesis.
Movement in the Phloem
- Plants also need to transport organic substances, e.g. carbohydrates produced in the leaves by photosynthesis, for respiration in other parts of the plant.
- Organic substances, also known as assimilates, are transported in the phloem; examples include sucrose, amino acids and plant hormones.
- Phloem sieve tube cells have reduced cell contents to reduce resistance to flow of assimilates, and sieve plates allow passage of assimilates between cells.
- Companion cells contain many mitochondria to produce ATP for the active loading of sucrose into the phloem tubes.
- Movement of assimilates through the phloem is called translocation.
- Translocation moves assimilates either upwards or downwards from cells in the source to cells in the sink.
- A source is the place in which assimilates are produced or stored, e.g. cells in photosynthesising leaves are a source of sugars; cells in storage organs during early spring may be a source of carbohydrates for new growth.
- A sink is the part of a plant where assimilates are required, e.g. cells in parts of a plant that are actively growing, or cells in plant storage organs.
- Translocation is an active process, dependent on energy from ATP.
The Mass Flow Hypothesis
- The process by which phloem sap moves in one direction along phloem sieve tubes is known as the mass flow hypothesis.
- The direction may differ depending on the location of sources and sinks in the plant.
- Sucrose loading: companion cells use ATP to actively pump hydrogen ions out of the cytoplasm into their cell walls; hydrogen ions move down their concentration gradient back to the cytoplasm via a cotransporter protein, carrying sucrose molecules; sucrose then moves into the sieve tubes via plasmodesmata.
- The high concentrations of solutes in the phloem lower the water potential and cause water to move into the phloem vessels by osmosis; water can move in from neighbouring xylem vessels.
- This results in increased hydrostatic pressure and generates a hydrostatic pressure gradient between the source and the sink; the contents of the phloem move towards the sink down a pressure gradient.
- At the same time sucrose is being unloaded from the phloem at the sink, lowering the water potential of the cells of the sink.
- Water follows by osmosis, maintaining the hydrostatic pressure gradient between the source and the sink.
- Movement of sucrose in the phloem is an active process, requiring energy from ATP to generate a hydrostatic pressure gradient.
Evaluating the Mass Flow Hypothesis
- The mass flow hypothesis is widely accepted, but it is a theory for which evidence is still being collected.
- Evidence for: when the phloem sieve tube is punctured, phloem sap oozes out, showing the contents exert pressure on the phloem walls.
- Evidence for: phloem sap extracted near a source has a higher sucrose concentration than sap extracted near a sink; water would move into the phloem by osmosis near a source and out near a sink.
- Evidence for: metabolic inhibitors stop translocation, showing ATP is required and it is an active process.
- Evidence for: removal of a ring of bark from trees results in a bulge above the ring, and fluid from the bulging region has a higher sugar concentration than fluid from below the ring; removal of the phloem tissue prevents the passage of sugars.
- Evidence against: amino acids appear to travel more slowly than sucrose in the phloem, but the hypothesis states they should flow at the same rate.
- Evidence against: experiments have detected different substances, within the same sieve tube, moving in opposite directions, but the hypothesis states everything should be flowing in one direction.
- Evidence against: sieve plates are present in the phloem, creating a barrier to mass flow, so there is no reason for them to have evolved.
Tracer and Ringing Experiments
- Tracers are chemicals that can be traced as they move through an organism; a common example is radioactive carbon dioxide, 14CO2.
- 14CO2 is readily absorbed by the leaves and used in photosynthesis to produce sucrose; the sucrose formed will be radioactive so its movement around the plant via translocation can be traced.
- Ringing experiments involve removing a ring of tissue from the outside of a plant stem.
- As the phloem is located towards the outside of the stem and the xylem towards the centre, the ring removes the phloem only, with the xylem remaining intact.
- In an example ringing experiment: plants were ringed at different locations on the stem, a control plant did not have a ring removed, plants were supplied with 14CO2, and after a period of time the levels of radioactive carbon in different parts of the plant were measured.
- Results show that the phloem is involved in the transport of sucrose, and not the xylem.
- No radioactive sucrose is detected past the ringing point on the stems, due to the break in the phloem at this point.
- In the phloem, the transport of sucrose occurs both upwards and downwards; sucrose is translocated from source tissues in the leaves to sink tissues above and below.
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இலவச முன்னோட்டம் — 61-இல் 8 கேள்விகள். அனைத்தையும் பார்க்க பதிவு செய்யவும்.
1.Which of the following is the best definition of transpiration?
Medium- AThe loss of water from the leaves of plants by evaporation
- BThe upward movement of water in the xylem
- CThe active transport of sugars into the phloem
- DThe movement of water from the soil into the roots by osmosis
2.Which of the following is a feature of xylem vessels that makes them specialised for the transport of water?
Medium- AThey contain many mitochondria to provide ATP for active transport
- BThey have no end walls and are hollow tubes
- CThey have sieve plates to allow passage of assimilates
- DThey have a large number of chloroplasts for photosynthesis
3.Which of the following are examples of assimilates transported in the phloem? (select all that apply)
Medium- ASucrose
- BAmino acids
- CPlant hormones
- DWater
- ELignin
4.Translocation is the movement of water and minerals in the xylem.
EasyTrue or false?
5.The mass flow hypothesis states that phloem sap moves in one direction along phloem sieve tubes.
EasyTrue or false?
6.Which of the following is evidence that supports the mass flow hypothesis?
Medium- AAmino acids travel more slowly than sucrose in the phloem
- BDifferent substances move in opposite directions within the same sieve tube
- CPhloem sap oozes out when a sieve tube is punctured
- DSieve plates are present in the phloem
7.Put the steps of the mass flow hypothesis in the correct order.
Medium- Sucrose is actively loaded into the phloem at the source
- Water enters the phloem by osmosis, increasing hydrostatic pressure
- A hydrostatic pressure gradient drives mass flow towards the sink
- Sucrose is unloaded from the phloem at the sink
8.Match each term with its correct description.
Medium- Transpiration
- Translocation
- Cohesion-tension theory
- Loss of water vapour from leaves
- Movement of assimilates in the phloem
- Explains upward water movement in xylem
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